1) After taking an aliquot of your cells and diluting them withTrypan Blue in a 1:1 dilution, you load the cell/Trypan mixtureonto a hemocytometer to count the cells.
You obtain the following counts:
| Quadrant | Transparent cells | Blue cells |
| 1 | 65 | 2 |
| 2 | 71 | 4 |
| 3 | 68 | 1 |
| 4 | 73 | 3 |
| 5 | 67 | 2 |
What is the average number of Trypan-diluted cells perquadrant?
2) Now, take into consideration the Trypan Blue dilution. Whatis the more concentrated number of cells per quadrant?
3) What is the volume in each quadrant of a hemocytometer?
4) What is the cell density (concentration) ofthe cells (cells/mL)?
5) If your original cell culture (the one you took an aliquotfrom to count) has 13 mL of cell suspension, what is thetotal number of cells you have in culture?
