1. An enzyme has a molecular weight of 25,000. If
Vmax= 80umol/min and you use 500uL...
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Chemistry
1. An enzyme has a molecular weight of 25,000. IfVmax= 80umol/min and you use 500uL of a 1mg/mL solutionof enzyme, what is the turnover number?
2. In an enzyme activity assay, a 100uL assay sample containing3.2 x 10-5 M enzyme produced an absorbance change perminute of 0.42. The molecular weight of the enzyme is 17,000 g/moland the extinction coefficient is 6220 M-1cm-1.
What is the activity of the enzyme?
What is the specific activity of the enzyme?
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3.9 Ratings (510 Votes)
1 Turnover number The units of sp act are moles unit time1 mg enzyme1 Here moles s1 mg1 is used and the value is 313 mole s 1 mg1 The turnover number kcat is effectively the activity in terms of mole active site1 instead of mg enzyme1 as in sp
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