1. An enzyme has a molecular weight of 25,000. IfVmax= 80umol/min and you use 500uL of a 1mg/mL solutionof enzyme, what is the turnover number?
2. In an enzyme activity assay, a 100uL assay sample containing3.2 x 10-5 M enzyme produced an absorbance change perminute of 0.42. The molecular weight of the enzyme is 17,000 g/moland the extinction coefficient is 6220 M-1cm-1.
What is the activity of the enzyme?
What is the specific activity of the enzyme?
