(1) Answer the three questions for each of the followingtranscription factors:
LacR
TrpR
CAP
GAL4
GR (glucocorticoid receptor)
(a) What is the small molecule thatcontrols its’ activity?
(b) In the presence of this molecule,is the protein on the DNA or off the DNA?
(c) What is the consequence fortranscription?
(2) Fill out the table with the words ON or OFF:
| - lactose: lac repressor ON or OFF theDNA | - lactose: lacZ ON or OFF | + lactose: lac repressor ON or OFF theDNA | + lactose: lacZ ON or OFF |
I+ O+ Z+ | | | | |
I- O+ Z+ | | | | |
I+ Oc Z+ | | | | |
I+ O+ Z+ / Oc | | | | |
I+ O+ Z+ / I- | | | | |
I- O+ Z+ / I+ | | | | |
I+ Oc Z+ / O+ | | | | |
(3) Answer the three questions for each of the followingenzymes:
Swi/Snf
HAT
HDAC
DNMT
(a) Describe the activity of each ofthe enzymes.
(b) What is the consequence of thisenzyme activity?
(c) How does this enzyme know whatgenes to act on?
