1. Calculate how to make 500.0 mL of 0.100 M triethanolaimesolution.
2. You need to prepare a buffer for biochemistry lab. Therequired solution is 0.5 M sodium phosphate, pH 7.0. Use theHenderson-Hasselbach equation to calculate the number of moles andgrams of monobasic sodium phosphate (NaH2PO4)and dibasic sodium phosphate (Na2HPO4)necessary to make 1 liter of the solution.
3. Dscribe how you would prepare a 0.1 M glycine buffer of pH10.0. You have available isoelectric glycine and sodium glycinate.Does it matter if you use sodium glycinate or potassium glycinatefor buffer? Why or why not?
4. Describe how you would prepare a 0.20 M Tris-HCl buffer of pH8.0. The only Tris reagent you have available is Tris base. Whatother reagent do you need, and how would you use it to prepare thesolution?
Please show all work. I appreciate the help!
