1. Can iodine be added before the primary stain in a Gram stain?Explain. If iodine was added before the primary stain, what wouldbe the final color of gram positive and gram negative cells in thestain and why?
2. Since you can’t identify bacteria from a Gram stain, whymight a physician perform a Gram stain on a sample beforeprescribing an antibiotic?
3. The cell envelope of Mycobacterium sp. is unlike the envelopeof other bacteria. While genetic evidence indicates that theseorganisms are related to gram-positive bacteria, one could arguethat their structural characteristics could place them in placethem among the gram-positives or the gram-negatives. Explain.
