1. Describe the principles behind and the applications of the
following:
a) Southern blotting
b) cDNA synthesis
c) CRISPR-Cas9...
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Biology
1. Describe the principles behind and the applications of thefollowing:
a) Southern blotting
b) cDNA synthesis
c) CRISPR-Cas9 gene editing
d) S1 Mapping or Primer Extension
e) Sanger Sequencing of DNA
f) Site-directed mutagenesis
j) cDNA cloning
k) Western Blotting
l) Restriction Enzymes
please answer them all because its including in one question!
Answer & Explanation
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1 a Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules This technique is mainly based on the principle of separation of DNA fragments by gel electrophoresis It is identified by labelled probe hybridization Southern blotting is useful for analysis of repetitive sequences because multiple similar sequences in the genome can be analyzed by a single probe Its quantitative results reflect the amounts of digested and undigested DNA molecules b Using mRNA as a template reverse transcriptase produces its complementary DNA cDNA based on the pairing of RNA base pairs cDNA can be obtained from both prokaryotes and eukaryotes The main advantage of cDNA is we can store the cDNA that was reverse transcribed from the RNA c A single RNA consisting of
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