1. Outline two experimental methods to test the hypothesis thatmarijuana alters a) gene expression and b) protein expression inmice brains. Explain also how you will know the specific identityof the genes and proteins that are altered. You will need to searchthe literature for biochemical techniques that would beappropriate.
2. Assume you find a gene that is upregulated in mice brains inresponse to marijuana. How would you determine exactly where in themouse brain this gene is expressed and to what extent? In addition,how would you determine the subcellular localization of the proteincorresponding to this gene? Briefly, outline five experiments toaddress these questions. You will need to search the literature forbiochemical techniques that would be appropriate.
