1. Retention time (tR) is a measure of how long a compound isretained on a stationaryphase in column in column chromatography.In TLC, Rf was a measure of how long thecompound was retained onthe stationary phase. What is the relationship between tR andRf?
2. What would be the order of elution if one separated thepigments of spinach on columnchromatography?
3. What if any would you have to change in terms of the mobilephase if you wanted toactually perform the column chromatography inorder to separate the pigments of spinachcompare to the one usedfor the TLC Experiment? Explain.
4. Why is it important to keep the solvent level above thestationary phase? What wouldhappen if one lets the solvent get muchlower than the alumina stationary phase?
