1. The blob operon produces enzymes that convert compound A intocompound B. The operon is controlled by a regulatory gene S.Normally, the enzymes are synthesized only in the absence ofcompound B. If gene S is mutated, the enzymes are synthesized inthe presence and in the absence of compound B. Does gene S producea regulatory protein that exhibits positive or negative control? Isthis operon inducible or repressible?
2.A mutation prevents the catabolite activator protein (CAP)from binding to the promoter in the lac operon. What will theeffect of this mutation be on transcription of the operon?
3.Compare the regulation of the lac operon and the effect on theproduction of ß-galactosidae when E. coli is grown under thefollowing conditions:
a) In the presence of high levels of lactose and absence ofglucose
b) In the presence of high levels of lactose and high levels ofglucose
