1. The mixed culture in an experimentwas prepared by mixing overnight E. coli and S.aureus cultures in the ratio 2:8. However, there seemed to bemore E. coli growth on the resulting streak plate thanS. aureus growth. Explain this. Â
2. It was observed that some coloniesof the same bacterial species appeared in different sizes on thestreak plates. Give the possible reason for this.
3. Is it better to prepare pureculture in broth or on agar plate?Explain.                 Â
