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  3. 1. there are three sets of assay data obtained fro

1. There are three sets of assay data obtained from a set of standard solution in...

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Biology

1. There are three sets of assay data obtained from a set ofstandard solution in an ELISA experiment (triplicate assay). UseExcel to draw a calibration curve. Then do the following:

(a) Calculate the Lower Limit of Detection (LLOD)

(b) Calculate the Limit of Quantitation (LOQ)

(c) Calculate the Sensitivity of the assay

(d) If an unknown sample gives an assay reading of 1.35. Use thecalibration curve to determine the concentration of thissample.

Concentration (ng/mL)

Assay1

Assay2

Assay3

0

0.04

0.02

0.03

0.1

0.05

0.03

0.04

0.5

0.15

0.18

0.16

1

0.25

0.29

0.27

2

0.50

0.48

0.52

5

1.20

1.10

1.18

10

1.98

1.89

2.20

20

3.95

3.90

4.20

Answer & Explanation Solved by verified expert
4.1 Ratings (451 Votes)
Since the curve is linear therefore the sensitivity is betweentwo linmitsa Calculate    See Answer
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