1. Two approaches for correcting single-gene defects are genetherapy such as is discussed in Section 3-5D and the CRISPR–Cas9system. Explain why the CRISPR–Cas9 approach can potentiallyprovide more complete restoration of normal tissue function thangene therapy.
2. A certain mutant DNA polymerase is error-prone, tending toincor-porate C opposite a template A. When such a DNA polymeraserepli-cates a segment of DNA containing an A · T base pair, whatwill be the DNA composition in the daughter cells after (a) one and(b) two rounds of cell division? Assume that DNA repair does notoccur.
3. Explain why mutations are more likely to occur on the laggingstrand than on the leading strand in eukaryotes.
