1. What are discrepancies between calf intestinal mucosaalkaline phosphatase and human alkaline phosphatase?
2. A solution was made up with p-Nitrophenyl phosphate (pNPP),0.1 M borate buffer at pH 9.5 and and alkaline phosphatase. Thissolution has good enzyme activity. Why does changing the pH to 9decrease the apparent activity of alkaline phosphatase?
3. solution was made up with p-Nitrophenyl phosphate (pNPP), 0.1M borate buffer at pH 9.5 and and alkaline phosphatase. 50 uM ofphosphate was added into the solution. Does the addition ofphosphate to the assay mix cause competitive or non-competitiveinhibition? Is this expected?
