Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. chemistry
  3. 1) when 10ug of an enzyme (mw 50,000) is added to

1) When 10ug of an enzyme (MW 50,000) is added to a reaction mixture containing its...

70.2K

Verified Solution

Question

Chemistry

1) When 10ug of an enzyme (MW 50,000) is added to a reactionmixture containing its substrate at a concentration one hundredtimes the Km, it catalyzes the conversion of 75umol of substrateinto product in 3 min. What is the enzyme's turnover number?

2) You want to load 15g of protein in 10L into one of the 12%polyacrylamide gel well. The protein needs to be in 1X buffer andin a total volume of 0.100 ml. You are given a 4.5 mg/ml proteinsolution, a 20X sample buffer, and distilled water. How much ofeach would you mix together to make required volume?

3) The Km of an enzyme of an enzyme-catalyzed reaction is 6.5uM.What substrate concentration will be required to obtain 45% of Vmaxfor this enzyme?

Answer & Explanation Solved by verified expert
3.8 Ratings (758 Votes)
Ans 1 Its assumed that at S Km velocity of enzyme catalysis is equal to its Vmax So Vmax rate of catalysis per unit time 75 umol 3 min 25 umol min1 Now using Turnover number Kcat Vmax ET    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Zin AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Q

1) One question discussed there: can we just give vaccines that show promise in animal studies/preclinical...

Medical Sciences
Q

A person presented with low back pain spreading along the distribution of S1 spinal nerve on...

Medical Sciences
Q

Which 1H NMR peaks will be key for discriminating (E)-stilbene from mesostilbene dibromide?

Chemistry
Q

How does personality expalin criminality?

Psychology
Q

A consumer electronics company is planning to introduce a new device. After careful consideration of costs,...

Advance Math
Q

India witnessed the launch of its biggest tax reform since Independence, the Goods and Services Tax...

Finance
Q

and move A particle of same mass m moving on the plane with velocity v...

Physics
Q

K M The following angle can be described as Select

Basic Math
Q

Calculate the binomial coefficient 2

Calculus
Q

1. Identify the total current assets and the total current liabilities of Walmart and calculate...

Accounting
Q

For the past several years, Steffy Lopez has operated a part-time consulting business from his...

Accounting
Q

Futura Company purchases the 73,000 starters that it installs in its standard line of farm...

Accounting