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1, Why use ninhydrin reagent and iodine crystals as visualizing agent in TLC? 2. Differentiate TLC and...

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Chemistry

1, Why use ninhydrin reagent and iodine crystals as visualizingagent in TLC?

2. Differentiate TLC and Column Chromatography based on a)separation of components in mobile and stationary phases b) solventmitigation c) type of support used

Answer & Explanation Solved by verified expert
3.6 Ratings (552 Votes)
Ninhydrin is the reagent used for the identification of amino acids The reagent is wellknown and widely used for its high sensitivity The identification of amino acids is important during the evaluation of protein structure The amino acids are colorless compound and it can be detected on the chromatogram by the spraying of Ninhydrin reagentIt reacts with amino acids and produces purple violet color with all amino acids But some exceptions are for proline and hydroxylproline due to the presence of secondary cyclic    See Answer
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