1) You are a research scientist studying a novel enzymeX, and you want to characterize this new enzyme. You measure thevelocity of the reaction with different substrate concentrationsand get the following data:
| [S] (mM) | Initial Velocity (mmol/min) |
| 3.0 | 10.4 |
| 5.0 | 14.5 |
| 10.0 | 22.5 |
| 30.0 | 33.8 |
| 90.0 | 40.5 |
a) Graph the above data. From the graph, estimate KMand Vmax (Michaelis-Menten Plot)
b) Use Lineweaver-Burk plot to calculate Km and Vmax.Show all equations and calculations.
c) You decide to do this experiment again, but thistime with only one third of the enzyme Xconcentration used in the first experiment. Draw a new graph on thesame graph that you did the first graph on. Estimate Km and Vmaxfrom the new graph.
