1) You have 100 ml of a solution of ATP. You take 25 ?l, anddilute it to a final volume of 1ml. The absorbance of the dilutedsolution at 259 nm is 0.550.
a) What is the ATP concentration in the original, undilutedsolution?
**The molar extinction coefficient for ATP at 259 nm is 15,600 lmol-1cm-1.
b. How many mg of ATP are there in total in the original,undiluted solution?
**The formula weight of ATP is 551.14 g/mole.
2) Fill in the blanks, with one of these 5 amino acids
Lysine/Cysteine/ Valine/ Tryptophan/Aspartate
a) If you dissolve (blank) in water, the resulting pH would beabout 8.5
b) if you dissolve (blank) in water, the resulting pH would beabout 4
c) (blank) residues would most likely be buried in the core of afolded protein structure
d) Collagen does not absorb strongly at 280 nm. This is becauseits sequence does not contain many (blank) residues.
e) (blank) can form disulfide bridges in proteins
3) You mix 100 ml of 0.1 M acetic acid with 100 ml of 0.1 Msodium acetate. The pKa of acetic acid is 4.76.
a) What is the pH of this mixture?
b) You then add 10 ml of 25 mM HCl. What will the new pH be?
c) What would the resulting pH be if you just added 10 ml of25mM HCl to 200 ml of distilled water?
