1) You have extracted your targeted plasmid from Milariuza.equilirus in your home. Because you do not have an expensivenanodrop machine, you decided to run a gel to determine theconcentration of your sample. However, you also don’t haveTris-acetate, thus can’t make any TAE buffer. Being a smartstudent, you realize you can simply make 1X TBE buffer instead of1X TAE buffer for your experiment. You will need 800ml TBE bufferfor both making the gel and use it as a running buffer. You do nothave any access to pipettes because you left them at school, andyou only have large graduate cylinders. This means that anymeasurement below 10ml is inaccurate. The balance you have can onlymeasure accurately above 100mg. Please show all works.
                                Recipe for 5X TBE buffer                  Stock Materials
500mMTris-Borate                               50M Tris-Borate
10mMEDTAÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â EDTA (MW ? 300)
5%NaOHÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â NaOH (MW ? 40)
