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10. Mutations in the lacI gene (lacI-) constitutively transcribe the lac operon. Mutations in the operator...

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Biology

10. Mutations in the lacI gene (lacI-) constitutively transcribethe lac operon. Mutations in the operator region can also occur,also resulting in the constitutive expression of the lac operon.Using the “reagents” described below, design an experiment to testif a mutant that constitutively expresses the lac operon is mutatedin the lacI gene or in the operator.
X-gal – a colorless chemical that is a substrate forB-galactosidase, when cleaved it produces a blue dye that “stains”any cells expressing B-galactosidase.
An F’ strain of E. coli that contains an F plasmid with afunctional lacI gene
An F’ strain that contains an F plasmid with a functional operatorsite
The F- mutant strain that constitutively expresses the lacoperon

You can make whatever media you want but must specify what mediayou use in each step.

Answer & Explanation Solved by verified expert
4.3 Ratings (592 Votes)
You need to perform two crosses Mutant F containing wildtype lacI gene Mutant F containing wildtype operator After each cross select Mutants containing F plasmid by antibiotic selection method in plate 1 F1 contains plasmid having wildtype lacI gene    See Answer
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