10. Mutations in the lacI gene (lacI-) constitutively transcribethe lac operon. Mutations in the operator region can also occur,also resulting in the constitutive expression of the lac operon.Using the “reagents” described below, design an experiment to testif a mutant that constitutively expresses the lac operon is mutatedin the lacI gene or in the operator.
X-gal – a colorless chemical that is a substrate forB-galactosidase, when cleaved it produces a blue dye that “stains”any cells expressing B-galactosidase.
An F’ strain of E. coli that contains an F plasmid with afunctional lacI gene
An F’ strain that contains an F plasmid with a functional operatorsite
The F- mutant strain that constitutively expresses the lacoperon
You can make whatever media you want but must specify what mediayou use in each step.
