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11. A package of yeast (7.4g) was suspended into 100 ml of water. The suspension was serially...

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Biology

11. A package of yeast (7.4g) was suspended into 100 ml ofwater. The

suspension was serially diluted by transferring 0.1 ml aliquotssuccessively to 9.9

ml of water three times in secession. 0.1 and 0.2 ml aliquotswere spread on

glucose nutrient agar. Colonies appeared as follows: 0.1 plate:179, 0.2 plate: 32

1. How many yeast were there originally in the package? How manyyeast

Were in a gram of material?

Please explain in detail and show all work.

Answer & Explanation Solved by verified expert
4.5 Ratings (884 Votes)
Dilution factor DF Initial volume Vi Final volume Vf In problem we diluted 01 ml aliquots to 99 ml of water Therefore DF 01 10 1 100 Here final volume 99 01 10 mL The    See Answer
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