1a) What are the differences between biological replicates andtechnical replicates? Explain. What are the appropriateapplications for each?
1b) Explain why statistical analysis of whole genome expressiondata (e.g., fpkm or tpkm from RNA-seq data) require using differentcriteria for statistical significance between experimental andcontrol samples than analyzing differences in expression of asingle gene (e.g., ΔCt or ΔΔCt values fromRT-qPCR) ?
1c) How have biologists addressed the concerns of part b?