2) You have 25 ml of an ATP solution You take 30 l and diluteto a fin...

2) You have 25 ml of an ATP solution. You take 30 Âµl and dilute to...

4.0 Ratings (743 Votes)

Absorbance = molar absorptivity x concentration x path length

assuming path length = 1 cm

we get,

concentration of diluted ATP solution = absorbance/molar absorptivity

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 0.432/15,400

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 2.80 x 10^-5 M

This is the concentration of 10 ml solution containing 30 uL (0.03 ml) of orginal solution

So,

concentration of original ATP solution = 2.8 x 10^-5 M x 10 ml/0.03 ml

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 9.33 x 10^-3 M

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â

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