3) a) Arguably, the way prokaryotic operons arestructured has evolutionary advantages. Based on our discussions inclass, briefly describe one advantage and one disadvantage of geneexpression regulation in prokaryotes. Provide appropriate examplesto strengthen your argument (3 pts).
b) Restriction enzymes, which are extensively used inmolecular biology, are products of bacterial “immune†system. Sincebacterial genomes span several million base pairs (E. coli > 4million bps), and the presence of restriction site within a genomeis more than likely, how does a bacteria manage to protect itselffrom innate REs? (1 pt )
3 d) Assuming the equal distribution of 4 nucleotideswithin a genome and a RE palindrome having C:G = A:T 6-baserecognition sequence, what is the likely size of the fragment ofthe DNA treated with this particular RE? (2 pts)
E) What is the difference between type I and type IIrestriction enzymes, and how would you utilize each in a molecularbiology experiment? Provide a simple hypothetical example for each.(3 pts)
