3) You have four tubes containing the same DNA at the sameconcentration in each tube (pH 7.5) but under differentenvironmental conditions (listed below). The TM for this DNAmolecule is 78°C at physiological salt conditions (~100 mM NaCl):a. DNA in an aqueous solution that has been digested completely tonucleotides with DNAse I enzyme. b. DNA in an aqueous solutioncontaining no salt and has been heated to 78°C. c. DNA in anaqueous solution containing physiological concentrations of saltand heated to 78°C. d. DNA in an aqueous solution containingphysiological concentrations of salt and is heated only to 37oC.Match the samples above with their corresponding OD260 readings.Next, explain your reasoning for each of the matches you made. (Forexample, “The DNA in sample (a) gives the highest OD readingbecause…â€). • OD260 = 0.620 • OD260 = 0.960 • OD260 = 0.475 • OD260= 0.271
