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  3. 3) you have four tubes containing the same dna at

3) You have four tubes containing the same DNA at the sameconcentrationin each tube (pH 7.5)...

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Biology

3) You have four tubes containing thesame DNA at the sameconcentrationin each tube (pH 7.5) but underdifferent environmental conditions (listed below).  TheTMfor this DNA molecule is 78°C at physiological saltconditions (~100 mM NaCl):

  1. DNA in an aqueous solution that has been digested completely tonucleotideswith DNAse I enzyme.

  1. DNA in an aqueous solution containing no salt and has beenheated to 78°C.

  1. DNA in an aqueous solution containing physiologicalconcentrations of salt and heated to 78°C.

  1. DNA in an aqueous solution containing physiologicalconcentrations of salt and is heated only to 37oC.

Match the samples above with theircorresponding OD260readings.  Next,explain your reasoning for each of the matches youmade.  (For example, “The DNA in sample (a)gives the highest OD reading because…”).

  • OD260= 1.01 à

  • OD260= 0.195à

  • OD260= 0.713à

  • OD260= 0.421à

Answer & Explanation Solved by verified expert
4.2 Ratings (730 Votes)
3 The melting temperature Tm is temperature at which the double stranded DNA will unwind and form a single stranded molecule When DNA is heated at or above its melting temperature under physiological conditions the DNA is denatured forming a single stranded molecule In this denatured form the nitrogenous bases will become unstacked Hence their absorbance will increase This is known as hyperchromatic effect a When DNA is completely digested with DNAse I in aqueous solution the OD260 nm is    See Answer
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