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34 Which of the following correctly describes an operon a Multiple genes organized behind one promoter the transcription of which results in a polycistronic mRNA b One regulatory protein capable of controlling expression of multiple genes throughout the genome c Short DNA sequences included within a gene that are removed after transcription d Regulatory sequences that are found in the middle of a gene 35 Which of the following is FALSE regarding DNA polymerase III a It requires a DNA template b It is responsible for synthesizing a new strand of DNA that is complementary to the template c It synthesizes the leading strand in the 5 3 direction and the lagging strand in the 3 5 direction d It has the ability to proofread its work increasing the overall accuracy of the product 36 Which of the following correctly states the events that occur during the initiation phase of replication a DnaA ATP binds to the 9 mer region which facilitates the melting of the nearby A T rich 13 mer region b DnaA ATP binds to the 9 mer region which facilitates the melting of the nearby G C rich 13 mer region c DnaA ATP binds directly to the A T rich 13 mer region after ATP is hydrolyzed the 13 mer region then denatures and DnaA ADP disengages from the origin so that replication can begin d DnaA ATP binds directly to the G C rich 13 mer region after ATP is hydrolyzed the 13 mer region then denatures and DnaA ADP disengages from the origin so that replication can begin 37 After replication RNA primers must be replaced with DNA Which of the following enzymes is NOT utilized during primer replacement a RnaseH b Ligase c DNA Polymerase I d Primase 38 While not being involved in the actual polymerization of DNA this protein is closely associated with DNA polymerase III and increases the processivity of the DNA polymerase III enzyme a Primase b Sliding clamp c Single stranded binding proteins d Helicase 39 Which option correctly identifies the order in which the following enzymes DNA polymerase III Helicase DnaA ATP and primase would be involved in DNA replication The enzymes should be in order from first to be utilized to the last a DnaA ATP primase helicase DNA polymerase III b helicase primase DnaA ATP DNA polymerase III c helicase DnaA ATP DNA polymerase III primase d DnaA ATP helicase primase DNA polymerase III

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