4. This is a coding DNA : 5’ ATG AGT GAT CTA GAC AGA CTT GCA AGCCGG …3’
(a) Predict the complementary strand and identify the strandname of it. (3' --> 5’)
(b) Predict the mRNA sequence (5' --> 3’) for the givencoding strand.
(c) Predict the amino acid sequence of the gene.
(d) Predict the amino sequence, if there is a base substitutionfrom A to T at the 16th base.
(e) Which type of mutation and protein will result from the basesubstitution?
(f) Identify the location(s) likely to be cut by the restrictionendonuclease XbaI, whose recognition site is5’…T^CTAGA…3’, and predict the digested DNA sequence (doublestranded).
