6.) In the 1952 Hershey-Chase experiment showed that DNA is thegenetic material of bacteriophage. Explain where the proteinlabeled with 35S ended up and where the DNA labeled with 32P endedup after centrifugation in their experiment. a. Draw a diagram ofthe experiment in support of your explanation. b. Is it possible todo the reverse labeling? (protein labeled with 32P and DNA islabeled with 35S) Why or why not?
7.) For each base-pair set, state if the two pairs aredistinguishable using the minor groove, the major groove, or both.a. G:C vs. C:G ________ b. A:T vs. G:C _________
c. A:T vs. T:A _________
8.) Ethidum bromide (EtBr) is a large flat, multiringed cation.Its flat shape allows it to insert itself between the stacked basepairs of double stranded (dsDNA). Because it fluoresces whenexposed to UV light and because its fluorescence increasesdramatically after intercalation, it is used as a stain tovisualize DNA. When ethidium intercalates it causes the DNA tounwind by about 26 degrees, reducing the normal rotation per basepair, decreasing the twist (T) of the DNA.
a. What effect would this change have on the writhe (W) of theplasmid?
b. When EtBr is used to visualize a relaxed covalently closedcircular plasmid by gel electrophoresis, what effect would thishave on the migration of the plasmid?
