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a b C Question How do acids and bases affect the reaction rate of an enzyme 2 The graph to the right shows the optimum pH for the enzyme catalase Remember that Acids have a pH of 1 6 9 and bases have a pH of 7 1 14 1 What is the optimum pH for this enzyme Hypothesis The following terms can be used to fill in the blanks below Terms may be used once more than once or not at all acidic basic speed up slow down 1 If we make the solution more acidic than water then the catalase reaction will Experiment Materials If we make the solution more basic than water then the catalase reaction will 3 reactions vials 3 cubes of potatoes exact same size crushed increasing enzyme activity Water Acetic acid vinegar Water sink Baking soda solution 5 6 optimum pH 7 pH Measured Measured height of the bubbles pH 8 Hydrogen peroxide Food coloring and dish soap to see the bubbles more easily Procedure 2 1 Obtain 3 reaction vials and 3 cubes of crushed potato Put the potato cubes in the vials Measure the pH of the vinegar water and baking soda with pH paper Record the values in the data table below 3 Add one to each of the three separate vials Pour just enough to cover the potato cube Acetic acid vinegar 9 10 4 Get approximately 30 ml of hydrogen peroxide in a beaker and add food coloring and dish soap a couple drops each 5 Pour 10 ml in each of the 3 reaction vials as quickly as you can and start a timer Mark the beginning height of the solution on each of the vials Environmental condition Acid vinegar Water Base baking soda solution Dispose of your waste and return the reaction vials and beakers where you found them 6 After 5 minutes mark the final height of the bubbles for each reaction on each of the vials 7 Calculate the difference in height for each of the reactions and record your data in the table below

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