A) David, an undergraduate research assistant in our lab,performed Western Blotting experiment and he used anti- Z proteinpolyclonal antibody raised in rabbit as a primary antibody. As asecondary antibody, he used anti-mouse HRP labeled antibody. Couldyou please tell him what is wrong with this experiment?
B) David, an undergraduate intern in our lab, forget to blockthe blot with BSA and continue to the antibody hybridization stepsand finally developed his blot. Could you please describe his blotpicture without even looking!
