a. How does regulated secretion differ from constitutivesecretion?
b. What would be the effect of mutating the KDEL sequence of aresident ER protein (like BiP)? Would this effect be similar ordifferent from that of mutating the KDEL receptor protein?
c.The pH of a compartment can be critical for association of areceptor and its cargo. How does the acidic pH of a late endosomeresult in different fates for the receptor and its cargo?
d. Why are carbohydrate groups of glycoproteins always exposedon the surface of the cell?
e.Compare and contrast the sorting of proteins to lysosomes tothe packaging of proteins into regulated secretory granules such asthose containing insulin.
f. How is a lysosomal protein targeted to a lysosome? Whateffect would be the addition of a lysosomal signal sequence have ofsubcellular localization of a protein that is normallycytosolic?  How would it affect localization of a proteinthat is normally secreted?
