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A) Imagine that you found a white colony on a LB plate (containing Ampicillin, X-gal, and...

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Biology

A) Imagine that you found a white colony on a LB plate(containing Ampicillin, X-gal, and arabinose) that did notfluoresce green E. coli. Propose a hypothesis for how this couldhave occured.

B) How could you test your hypothesis using techniques andequipment in a lab.

Answer & Explanation Solved by verified expert
4.4 Ratings (1090 Votes)
AHYPOTHESISpGLOLB plate the control Without the pGLO plasmid thebacteria inside the plate will grow since the bacteria areresistant to the antibiotics However the bacteria will not glowin the dark since there is no pGLO plasmid inside the platepGLOLBamp plate Inside this plate the bacteria will neithergrow nor glow since there is antibiotic to stop the growth of thebacteria and pGLO plasmid is not presented in the platepGLOLBamp plate For this plate the bacteria will grow yetnot glowpGLOLBampara plate As this plate has LB amp and arabinosea type of sugar The bacteria will both grow andglowBMETHODOLOGYMATERIALS1 EColi starter plate4 agar plates labeled as suchpGLOLBamppGLOLBamparapGLOLBpGLOLBamp4 microtubules1microtubule containing transformation solution1microtubule containing LB broth1 emptymicrotube labeled 1 emptymicrotubule labeled A foam microtubule holder1 container with ice water1 package of sterile inoculation loops4 sterile pipettes1 micropipette 200 1000 ul and micropipette tips1 micropipette 20 200 ul and micropipette tips1 Sharpie1 UV lightlampPROCEDURE1Labelone closed micro test tube pGLO and another pGLO Label bothtubes with your groups name Place them in the foam tube    See Answer
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