A protein assay requires the reaction of the protein solutionwith a protein-binding dye for one minute, followed by measuringthe absorbance of the protein-dye complex at 505 nm. The absorbancedata for four standard protein solutions and the blank is providedin the table below. The absorbance of each solution was measuredthree times. Construct a calibration curve using the data in thetable, and find the slope (m), intercept (b), and their respectivestandard deviations using the method of least squares. Note thatthe calibration curve will be created from absorbance valuescorrected for the absorbance of the blank and not the raw data inthe table (including individual blank values). When creating thecalibration curve, do not use the average absorbance at eachprotein concentration. Instead, use each data point separately,meaning there will be three data points at each proteinconcentration, for a total of 15 points (including each individualblank value).
| replicate 1 | replicate 2 | replicate 3 |
| blank | 0.0332 | 0.0331 | 0.0335 |
| 0.10 g/dL | 0.1002 | 0.0999 | 0.998 |
| 0.20 g/dL | 0.233 | 0.233 | 0.233 |
| 0.50 g/dL | 0.532 | 0.534 | 0.533 |
| 1.00 g/dL | 1.032 | 1.033 | 1.031 |
m= 1.012 +/- ?
b= ? +/- ?
Calculate the concentration and uncertainity of a proteinsolution that produced an averge absorption of 0.470 when measuredthree times.
number = ? +/- ?
