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A researcher wants to clone genes using recombinant gene technology as part of a genome sequencing...

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Biology

  1. A researcher wants to clone genes using recombinant genetechnology as part of a genome sequencing project. There are eightsteps below. First, cross out the two steps that are notpart of this process. Then, put the remaining six steps in thecorrect order.

A Insert recombinant plasmid into bacterium.

B Cut the genome into fragments using restriction enzymes.

C Determine activity using laser.

D Use DNA Ligase.

E Make cDNA using reverse transcriptase.

F Grow bacteria and add clone to genomic library.

G Cut bacterial plasmids with restriction enzymes.

H Combine DNA fragments and cut plasmid DNA.

Answer & Explanation Solved by verified expert
4.2 Ratings (675 Votes)
The following forms the reactions1 Cut the bacterial Plasmid with a restriction enzyme forfurther reaction or cloning purpose2 Cut the genome into fragments using restriction enzyme3 Use DNA ligase4Combine DNA fragment    See Answer
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