a start up company is trying to produce its own brand ofantibacterial ointment. it decides to analyze a generic brandalready on the market so that it can make suitable modification tothe chemical composition. its known that the generic ointmentcontain benzoic acid( active ingredient), benzhydrol (the fixative)and anise, which add fragrance. you are a part of development teamthat wishes to separate these three substances in order todetermine their relative proportions in the ointment, as well as todiscover any inactive or unlisted constituents. some member of yourteam were able to isolate the inactive ingredient; However, NMRreveals that you still have a mixture of benzoic acid, benzhydrol,anisole left unseparated. as a member of team, you have been askedto do the following: 1. design an experiment to separate thefollowing compounds from a mixture using any combination ofpurification techniques (TLC, column chromatography, Extraction andrecrystallization) 2. carry out characterization studies such asmelting point, IR, NMR to confirm that your purification wassuccessful. 3. determine the percent recovery of the individualcomponents of the mixture. mixture originally contained; 0.5gbenzoic acid, 0.25g benzhydrol, 0.25ml anisole and 10ml diethylether.
