A strain of E. coli carries a mutation thatcompletely inactivates the enzyme encoded in the gene. Severalrevertants, mutants of the mutant with partly or fully restoredactivity, were selected, and the amino acid sequence of the enzymewas determined. The only differences found were at position 10 inthe polypeptide chain.
Revertant 1 had Thr.
Revertant 2 had Glu.
Revertant 3 had Met.
Revertant 4 had Arg.
Assume that the initial mutation itself, as well as eachrevertant, resulted from a single nucleotidesubstitution.
a. What amino acid is present at position 10 in themutant protein?
b. What codon in the mRNA would encode this aminoacid?
Please provide explanations and not just correctanswers.
