a. You start a culture of stem cells and you use a protocol todifferentiate them into beta cells. To make sure they are trulybeta cells.
First you want to check whether the insulin gene is expressed athigher levels in the differentiated vs. the undifferentiated cells.What methods that we studies would you use and describe the step bystep procedure.
b. Then you want to demonstrate that they secrete insulin whenyou place glucose in their culture media. What methods that westudies would you use and describe the step by step procedure.
c. Finally, you want to show that inside differentiated cellsinsulin is located within secretory vesicles and quantify in whichpercentage of the cells. What methods that we studies would you useand describe the step by step procedure.
