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After extracting DNA in 5 warbler birds and running a PCR with one microsatellite primer,...

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After extracting DNA in 5 warbler birds and running a PCR with one microsatellite primer, the following gel image was produced. Answer the following questions based on the gel image. (L is the DNA ladder lane) (3) a) How many of the five birders are homozygous? (0.5) b) i. What is the allele frequency (p) of the large band in these results? (0.5) ii. How many heterozygous individuals would you expect to see in this small sample of individuals, if it is in Hardy-Weinberg equilibrium? (Show all calculations.) (1.5) c) You want to estimate the inbreeding coefficient for this population. Which individuals of the 5 warblers would you be most interested in? (0.5) 1 2 3 4 5 6

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