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An enzyme was found that catalyzes the reaction between students, S and academic success, P (for...

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Chemistry

An enzyme was found that catalyzes the reaction betweenstudents, S and academic success, P (for pass); the enzyme wascalled studyase.

An enzyme assay was run on studyase. 10μg of studyase (molecularweight 20,000D) was placed in a set of test tubes each of whichcontained 1mL of the substrate student at varying concentrationsand the amount of product, academic success (P) was determined.

The following results were determined:

S, (mM)

Velocity, V (μmole/mL/sec)

1

12

2

20

4

29

8

35

12

40

Note 1mM = 1millimole/L = 1μmol/mL

Plot a Lineweaver-Burk plot of 1/V versus 1/S

Determine Vmax and Km for the enzyme studyase.

Calculate Kcat and the catalytic constant (kcat/km) for theenzyme. (find [E]T as total μmoles/mL)

Another enzyme called partyase was analyzed and when 0.010μmoles of this enzyme was assayed it was found to have a Vmax ofabout 50 μmol/mL/sec and a Km of 5 mM. Calculate Kcat and thecatalytic constant for this enzyme.

i.Compare the 2 enzymes on the basisof affinity for the binding site and kinetic efficiency.

Answer & Explanation Solved by verified expert
4.4 Ratings (823 Votes)
a Lineweaver Burk plotb For the above graph1Vmax Yintercept 0019 Vmax 10019 526 mMs or micromolmLs answerKm Vmax slope 0063 s Km 0063 s x Vmax 0063 s x 526 mMs    See Answer
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