Analytical Chemistry - Determination of Fe in Vitamintablet:
Iron was reduced by 25 mL 6M HCl and DI water.
Unknown Iron Sample: 10 mL diluted with DI water in 100 mLvolumetric flask,
Iron Standards: 30 mL of 0.7385 mM
2 mL of hydroquinone solution, 3 mL o-phenanthroline solutionand 10 mL of pH 3.5 buffer added to all flasks.
| Blank (100 mL volumetric flasks) | flask 1 | flask 2 | flask 3 | flask 4 | |
0 mL Standard 50 mL DI Water | 1 mL standard 10 mL Unknown | 2 mL standard 10 mL Unknown | 5 mL standard 10 mL Unknown | 10 mL standard 10 mL Unknown | |
All flasks were diluted to the 100 mL mark. Spectroscopy wasused to measure the absorbance at 510 nm and values of wavelengthand absorbance for 6 runs are known.
Please explain in detail how to plot a Beer-lambert law from mywavelength and absorbance data in excel. How to plot the absorbancevs. iron concentration added in the standards (in μg) and calculatethe μg of iron in the standards and determine the unknownconcentration from the curve?
