Assignment:
1) Describe the principle of the yeast-two hybrid system
2) A positive result for the yeast two-hybrid analysismeans:
A. two fusion proteins, one containing the Gal4p binding sitedomain and one containing the Gal4p activation domain, haveinteracted through their Gal4p domains resulting in transcriptionof the reporter gene.
B. two fusion proteins, one containing the Gal4p binding sitedomain and one containing the Gal4p activation domain, have boundtogether, which disrupts the ability of the reporter gene to make ablue colony.
C. two fusion proteins interact with each other and because theGal4p binding site domain on one fusion protein binds to thereporter gene and the Gal4p activation domain of the other fusionprotein interacts with RNA polymerase, the reporter gene isactivated.
D. two fusion proteins interact with each other and because ofthe Gal4p binding site domain and the Gal4p activation domaincontained in each fusion protein, the reporter genes are activatedand the cells die, resulting in no colonies. E. the coloniesgrowing are expressing the two fusion proteins and the two proteinshave functioned independently.
3) What could the reporter gene encode and how would you detectreporter gene expression?
