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Assume that the gene trpA in an auxotrophic strain of E. coli is located at 27...

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Biology

Assume that the gene trpA in an auxotrophic strain ofE. coli is located at 27 minutes, whereas the genepyrE is located at 81 minutes.
Present an experimental scheme that would allow you to convert oneof the auxotrophic strains to a prototrophic strain.

A. F+ (auxotroph) × F− (wild type)

B. Hfr (auxotroph) × F− (auxotroph)  

C. Hfr (auxotroph) × F− (wild type)

D. Hfr (wild type) × F−(auxotroph)

E. F+ (wild type) × F− (auxotroph)

The correct answer is D, but I don't understand why.Could someone explain this? Thank you!

Answer & Explanation Solved by verified expert
4.3 Ratings (591 Votes)
Before I explain why option D is correct you need to be given an understanding of what auxotrophic and prototrophic strains mean So an auxotrophic strain can be seen as a mutant    See Answer
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