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Because electron transport is coupled to ADP phosphorylation, it is theoretically possible to drive electron backwards...

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Biology

Because electron transport is coupled to ADP phosphorylation, itis theoretically possible to drive electron backwards in the chain,against the potential gradient, at the expense of ATP. Thismechanism has been observed in some organisms. Consider a bacteriumat 25 C that can transfer electrons from succinate to NAD+ by meansof the transport

Succinate + NAD+ = Fumerate + NADH + H+

If the intracellular concentration of phosphate is constant at10 mM, calculate the [ATP]/[ADP] ratio that would be required tomaintain the steady-state ratio of [NADH]/[succinate] = 0.010.Assume that the intracellular concentrations of fumerate and NAD+are equal.

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4.2 Ratings (706 Votes)

Succinate + NAD+ = Fumerate + NADH + H+

ATP hydrolysis takes place during this reaction,

ATP = ADP + Pi

Given: Steady state reaction, phosphate is constant at 0.01 M, [NADH]/[succinate] = 0.01 M

Rate = [ADP] [Pi] = [FUMARATE] [NADH]  

            ATP              [NAD+] [SUCCINATE]

Hence: NAD+ = FUMARATE, and [NADH]    = 0.01 M

                                                  [SUCCINATE]

So, [ADP] [Pi] = 1 (ie, 0.01 divided by 0.01)

        ATP

Rearrange: [ATP] = 1
                 [ADP] [Pi]
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