BioChemistry Problem
State whether the Hill Coefficient (N) is positive or negative?Explain your reasoning in each case.
(a) The protein has multiple subunits, each with a singleligand-binding site. Binding of ligand to one site increases thebinding affinity of other sites for the ligand.
(b) The protein is a single polypeptide with two ligand-bindingsites, site one causes a structural change that decreases affinityfor the second ligand.
(c) A molecule binds to a protein and inhibits binding of thenatural ligand.
(d) The protein has two subunits and as one ligand binds, thesecond binds with lower affinity.
