Can Western blotting be used as a quantitative technique? How could we use Western analysis to...

70.2K

Verified Solution

Question

Biology

Can Western blotting be used as a quantitative technique? Howcould we use Western analysis to determine the relative change in aprotein's expression levels between two treatments? What would weneed to be able to determine the absolute level of a protein (i.e.ng/mg of total protein)?

Answer & Explanation Solved by verified expert

3.9 Ratings (386 Votes)

Western Blot is a technique that is used to separate on a polyacrylamide on the basis of their size This technique can also be exploited to quantify the amount of proteins present in a sample However this cannot be done directly and an See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member

Can Western blotting be used as a quantitative technique? How could we use Western analysis to...

70.2K

Verified Solution

Question

Biology

Can Western blotting be used as a quantitative technique? Howcould we use Western analysis to determine the relative change in aprotein's expression levels between two treatments? What would weneed to be able to determine the absolute level of a protein (i.e.ng/mg of total protein)?

Answer & Explanation Solved by verified expert

Get Answers to Unlimited Questions

Membership Benefits:

Other questions asked by students

A cookie company wants to expand its retail operations. Based on a preliminary study, 10 stores...

what sections of the code deal with using pirated / illegal software ? Principle 6: Profession Software engineers...

Demand for propane is given by D(x) = 6.5 ? 0.25x, and supply is given by...

A spring of spring constant 5 x 10 N m is stretched initially by 5...

3 Two cylinders of same cross section and length L but made of two different...

Each student's score on the final exam in Mr. Shenton's class58, 72, 74, 92, 84,...

Find an equation of the tangent line to the parabola y x at the point...

A particle moves along a line so that at time t where 0 t its...

3. Breakdown of a cartel agreement Consider a town in which only two residents, lake...

6.00 points Exercise 13-17 Opportunity cost LO 13-5 Brewton Freight Company owns a truck that...

Need help with this problem Paulson Company issues 8%, four-year bonds, on January...

Assume that the activities in Problem 3.15 (see below) have the following costs to shorten:...