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Chymotrypsin activity was measured by the hydrolysis of p- nitrophenylacetate Answer following questions 1.1. What are the...

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Biology

Chymotrypsin activity was measured by the hydrolysis of p-nitrophenylacetate Answer following questions
1.1. What are the catalytic triad amino acid residues? 1.2. Inthe reaction mechanism, what amino acid is the residue initiatingthe reaction mechanism, and explain how other amino acids areinvolved in H-bonding and acyl bond formation with the enzyme
1.3. draw the structure of tetrahedral intermediate of thesubstrate,
1.4. what is oxyanion hole in the active site and its role inthe catalysis
1.5. explain how an water molecular is used as anothersubstrate

Answer & Explanation Solved by verified expert
3.9 Ratings (409 Votes)
11catalytic triad amino acid residuesSerine195 Histidine57 Aspartate102number depict sequence number12 Serine is the residue initiating the reaction    See Answer
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