Compare and contrast Native -PAGE, SDS-PAGE, size-exclusion
chromatography, and affinity chromatography, ion exchange
chromatography. How are components...
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Chemistry
Compare and contrast Native -PAGE, SDS-PAGE, size-exclusionchromatography, and affinity chromatography, ion exchangechromatography. How are components separated by these proceduresand how are they detected? Explain details. hHow are each of thesetechniques carried out?
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NativePAGE NativePage is a type of Polyacrylamide gel electrophoresis PAGE technique used for separation of biological macromolecules such as proteins and nucleic acid in their native form Macromolecules in their native state preserve the highest order structure and they are separated based on their electrophoretic mobility which depends upon length conformation and charge on the molecule This technique involves preparation of gel of polyacrylamide After application of sample on this gel electricity is applied across the gel so that positively charged biomolecules move towards negative electrode and vice versa Small molecules move faster
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