Compare the primary role of Mg2+ [ Mg(II)] in the
mechanism of restriction endonucleases with that...
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Biology
Compare the primary role of Mg2+ [ Mg(II)] in themechanism of restriction endonucleases with that of Zn2+in the carbonic anhydrases.
Explain the role of Ser 236 in abstracting a proton from theattacking water and giving up a proton to the Mg2+-ATPcomplex.
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Carbonic anhydrases catalyze the reaction of water with carbon dioxide to generate carbonic acid The catalysis can be extremely fast molecules of some carbonic anhydrases hydrate carbon dioxide at rates as high as 1 million times per second A tightly bound zinc ion is a crucial component of the active sites of these enzymes Each zinc ion binds a water molecule and promotes its deprotonation to generate a hydroxide ion at neutral pH This hydroxide attacks carbon dioxide to form bicarbonate ion HCO3 Because of the physiological roles of carbon dioxide and bicarbonate ions speed is of the essence for this enzyme To overcome limitations imposed by the rate of proton transfer from the
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