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Consider an LB broth flask into which two E. coli strains are inoculated. Strain \"A\" is...

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Biology

Consider an LB broth flask into which two E. coli strains areinoculated. Strain \"A\" is wild-type, with g = 22 min at 37oC, and45 min at 27oC. Strain \"B\" has a temperature-sensitive mutation,such that g = 52 min at 37oC, but at 27oC g = 44 min.We inoculateequal amounts of cells (1 ml of each starter culture whosepopulation is 1 x 106 cfu/ml) into each of two separate 1 litervolumes of LB broth at time = 0. One of the flasks is incubated at37oC, one at 27oC.Assuming constant exponential growth (i.e. no lagphase, and no exhaustion of nutrients), answer each of thesequestions:

1) After 6 hours of growth, what is the final concentration ofeach strain in each flask?

2) What fraction of the total population is Strain A and StrainB in each flask?

Answer & Explanation Solved by verified expert
3.6 Ratings (635 Votes)
1 Final concentration of each strain in each flask Time 6 hours 660 360 minutes Concentration of starter culture Xo 1106 CFUml Final concentration Xo 2Timeg i 37oC Strain A Generation time g 22 minutes Final concentration 1 106 236022 1 106 21636 84111 1010 Final concentration of strain A 84111 1010 CFUml    See Answer
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