Consider the mitochondrial electron transport chain thatutilizes NADH
a. Write a NET balanced equation for the entire reaction run byComplex I, Complex III and Complex IV together. Include the protonspumped from the inside to the outside of the matrix.
b. If you assume that the membrane potential is 200 mV and thepH gradient is 0.5 and the temperature is 37 C, how much energy isstored by the total reaction you wrote down in (a) as a membraneproton motive force per mole of NADH consumed?
c. Now write down the two half reactions that make up thereaction from part (a) (ignoring the protons pumped).
d. Using the values for E0 in the book for these reactions, whatis the standard energy of the reaction you wrote down in (a)? e.What fraction of the total oxidation energy of NADH is stored asATP. Assume that the reaction ADP + Pi à ATP + H2O has a freeenergy of 50 kJ/mole and assume the actually free energy of NADHoxidation is the same as the standard free energy you calculatedabove.
Please show work for a-d thank you.
